Journal: Bio-protocol
Article Title: Isolation and Characterization of Live Yeast Cells from Ancient Clay Vessels
doi: 10.21769/BioProtoc.3473
Figure Lengend Snippet: A. several types of yeast colonies isolated from ancient vessels ( Aouizerat et al., 2019 ). In general, yeast colonies are relatively large with a shiny white color in contrast to bacterial colonies which are usually small and matte. B. Yeast cells observed under light microscopy (60x). Note that yeast cells are usually bigger than typical bacteria and many kinds of yeast divide by budding rather than fission. C. Upper panel, a selective agar plate for fungal isolation was plated as described in the Procedure A of Part II. Note the low number of bacterial colonies due to the antibiotics (blue circle), structure of molds (green circle) and distinctive shiny colony of yeast (red circle). In the bottom panel, a yeast colony which was picked and re-streaked on a YPD plate (red arrow).
Article Snippet: Collect any colony which seems to be a yeast colony, observe for typical yeast cells using light microscopy ( ) and plate it on solid YPD agar plates, containing 2% w/v Bacto-agar ( ).
Techniques: Isolation, Light Microscopy, Bacteria